Search results for "Zona free"

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Vitrification of zona-free rabbit expanded or hatching blastocysts: a possible model for human blastocysts.

2003

BACKGROUND: The purpose of this study was to test the effectiveness of one two-step (A) and two one-step (B1 and B2) vitrification procedures on denuded expanded or hatching rabbit blastocysts held in standard sealed plastic straws as a possible model for human blastocysts. The effect of blastocyst size was also studied on the basis of three size categories (I: diameter <200 μm; II: diameter 200-299 μm; III: diameter ≥300 μm). METHODS: Rabbit expanded or hatching blastocysts were vitrified at day 4 or 5. Before vitrification, the zona pellucida was removed using acidic phosphate buffered saline. For the two-step procedure, prior to vitrification, blastocysts were pre-equilibrated in a solut…

medicine.medical_specialtyHot TemperatureZona freeBiologyAndrologychemistry.chemical_compoundEmbryonic and Fetal DevelopmentmedicineAnimalsHumansVitrificationBlastocystZona pellucidaZona PellucidaCryopreservationTissue SurvivalDimethyl sulfoxideHatchingRehabilitationHistological TechniquesObstetrics and GynecologyEmbryoSurgerymedicine.anatomical_structureBlastocystReproductive MedicinechemistryModels AnimalFemaleRabbitsEthylene glycolHuman reproduction (Oxford, England)

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PEPhysical Sciences and Engineering
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